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Archaeal Orc1/Cdc6 Proteins

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The Eukaryotic Replisome: a Guide to Protein Structure and Function

Part of the book series: Subcellular Biochemistry ((SCBI,volume 62))

Abstract

The initiation of DNA replication in most archaeal genomes is mediated by proteins related to eukaryotic Orc1 and Cdc6. Archaeal replication origins have been mapped and their interactions with Orc1/Cdc6 proteins have been characterized at the biochemical level. Structural and biophysical studies have revealed the basic rules of sequence recognition by archaeal initiators.

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References

  • Bell SD (2011) DNA replication: archaeal oriGINS. BMC Biol 9:36

    Article  PubMed  CAS  Google Scholar 

  • Bell SP, Dutta A (2002) DNA replication in eukaryotic cells. Annu Rev Biochem 71:333–374

    Article  PubMed  CAS  Google Scholar 

  • Brochier-Armanet C, Boussau B, Gribaldo S, Forterre P (2008) Mesophilic crenarchaeota: proposal for a third archaeal phylum, the Thaumarchaeota. Nat Rev Microbiol 6:245–252

    Article  PubMed  CAS  Google Scholar 

  • Capaldi SA, Berger JM (2004) Biochemical characterization of Cdc6/Orc1 binding to the replication origin of the euryarchaeon Methanothermobacter thermoautotrophicus. Nucleic Acids Res 32:4821–4832

    Article  PubMed  CAS  Google Scholar 

  • Dueber ELC, Corn JE, Bell SD, Berger JM (2007) Replication origin recognition and deformation by a heterodimeric archaeal Orc1 complex. Science 317:1210–1213

    Article  PubMed  CAS  Google Scholar 

  • Dueber EC, Costa A, Corn JE, Bell SD, Berger JM (2011) Molecular determinants of origin discrimination by Orc1 initiators in archaea. Nucleic Acids Res 39:3621–3631

    Article  PubMed  CAS  Google Scholar 

  • Duggin IG, McCallum SA, Bell SD (2008) Chromosome replication dynamics in the archaeon Sulfolobus acidocaldarius. Proc Natl Acad Sci USA 105:16737–16742

    Article  PubMed  CAS  Google Scholar 

  • Edgell DR, Doolittle WF (1997) Archaea and the origin(s) of DNA replication proteins. Cell 89:995–998

    Article  PubMed  CAS  Google Scholar 

  • Elkins JG, Podar M, Graham DE, Makarova KS, Wolf Y, Randau L, Hedlund BP, Brochier-Armanet C, Kunin V, Anderson I, Lapidus A, Goltsman E, Barry K, Koonin EV, Hugenholtz P, Kyrpides N, Wanner G, Richardson P, Keller M, Stetter KO (2008) A korarchaeal genome reveals insights into the evolution of the Archaea. Proc Natl Acad Sci USA 105:8102–8107

    Article  PubMed  CAS  Google Scholar 

  • Erzberger JP, Berger JM (2006) Evolutionary relationships and structural mechanisms of AAA plus proteins. Annu Rev Biophys Biomol Struct 35:93–114

    Article  PubMed  CAS  Google Scholar 

  • Erzberger JP, Mott ML, Berger JM (2006) Structural basis for ATP-dependent DnaA assembly and replication-origin remodeling. Nat Struct Mol Biol 13:676–683

    Article  PubMed  CAS  Google Scholar 

  • Evrin C, Clarke P, Zech J, Lurz R, Sun JC, Uhle S, Li HL, Stillman B, Speck C (2009) A double-hexameric MCM2-7 complex is loaded onto origin DNA during licensing of eukaryotic DNA replication. Proc Natl Acad Sci USA 106:20240–20245

    Article  PubMed  CAS  Google Scholar 

  • Gaudier M, Schuwirth BS, Westcott SL, Wigley DB (2007) Structural basis of DNA replication origin recognition by an ORC protein. Science 317:1213–1216

    Article  PubMed  CAS  Google Scholar 

  • Grainge I, Gaudier M, Schuwirth BS, Westcott SL, Sandall J, Atanassova N, Wigley DB (2006) Biochemical analysis of a DNA replication origin in the archaeon Aeropyrum pernix. J Mol Biol 363:355–369

    Article  PubMed  CAS  Google Scholar 

  • Iyer LM, Leipe DD, Koonin EV, Aravind L (2004) Evolutionary history and higher order classification of AAA plus ATPases. J Struct Biol 146:11–31

    Article  PubMed  CAS  Google Scholar 

  • Jacob F, Cuzin F, Brenner S (1963) On regulation of DNA replication in bacteria. Cold Spring Harb Symp Quant Biol 28:329–348

    Article  CAS  Google Scholar 

  • Liu JY, Smith CL, DeRyckere D, DeAngelis K, Martin GS, Berger JM (2000) Structure and function of Cdc6/Cdc18: implications for origin recognition and checkpoint control. Mol Cell 6:637–648

    Article  PubMed  CAS  Google Scholar 

  • Lundgren M, Andersson A, Chen LM, Nilsson P, Bernander R (2004) Three replication origins in Sulfolobus species: synchronous initiation of chromosome replication and asynchronous termination. Proc Natl Acad Sci USA 101:7046–7051

    Article  PubMed  CAS  Google Scholar 

  • Majernik AI, Chong JPJ (2008) A conserved mechanism for replication origin recognition and binding in archaea. Biochem J 409:511–518

    Article  PubMed  CAS  Google Scholar 

  • Matsunaga F, Takemura K, Akita M, Adachi A, Yamagami T, Ishino Y (2010) Localized melting of duplex DNA by Cdc6/Orc1 at the DNA replication origin in the hyperthermophilic archaeon Pyrococcus furiosus. Extremophiles 14:21–31

    Article  PubMed  CAS  Google Scholar 

  • Myllykallio H, Lopez P, Lopez-Garcia P, Heilig R, Saurin W, Zivanovic Y, Philippe H, Forterre P (2000) Bacterial mode of replication with eukaryotic-like machinery in a hyperthermophilic archaeon. Science 288:2212–2215

    Article  PubMed  CAS  Google Scholar 

  • Norais C, Hawkins M, Hartman AL, Eisen JA, Myllykallio H, Allers T (2007) Genetic and physical mapping of DNA replication origins in Haloferax volcanii. PLoS Genet 3:729–743

    Article  CAS  Google Scholar 

  • Remus D, Beuron F, Tolun G, Griffith JD, Morris EP, Diffley JFX (2009) Concerted loading of Mcm2-7 double hexamers around DNA during DNA replication origin licensing. Cell 139:719–730

    Article  PubMed  CAS  Google Scholar 

  • Robinson NP, Bell SD (2007) Extrachromosomal element capture and the evolution of multiple replication origins in archaeal chromosomes. Proc Natl Acad Sci USA 104:5806–5811

    Article  PubMed  CAS  Google Scholar 

  • Robinson NP, Dionne I, Lundgren M, Marsh VL, Bernander R, Bell SD (2004) Identification of two origins of replication in the single chromosome of the Archaeon Sulfolobus solfataricus. Cell 116:25–38

    Article  PubMed  CAS  Google Scholar 

  • Robinson NP, Blood KA, McCallum SA, Edwards PAW, Bell SD (2007) Sister chromatid junctions in the hyperthermophilic archaeon Sulfolobus solfataricus. EMBO J 26:816–824

    Article  PubMed  CAS  Google Scholar 

  • She Q, Singh RK, Confalonieri F, Zivanovic Y, Allard G, Awayez MJ, Chan-Weiher CCY, Clausen IG, Curtis BA, De Moors A, Erauso G, Fletcher C, Gordon PMK, Heikamp-de Jong I, Jeffries AC, Kozera CJ, Medina N, Peng X, Thi-Ngoc HP, Redder P, Schenk ME, Theriault C, Tolstrup N, Charlebois RL, Doolittle WF, Duguet M, Gaasterland T, Garrett RA, Ragan MA, Sensen CW, Van der Oost J (2001) The complete genome of the crenarchaeon Sulfolobus solfataricus P2. Proc Natl Acad Sci USA 98:7835–7840

    Article  PubMed  CAS  Google Scholar 

  • Singleton MR, Morales R, Grainge I, Cook N, Isupov MN, Wigley DB (2004) Conformational changes induced by nucleotide binding in Cdc6/ORC from Aeropyrum pernix. J Mol Biol 343:547–557

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

I thank Marek Drozdz and Rachel Samson for their comments on this chapter. I would also like to thank Alessandro Costa for valuable discussions on the role of ATP in these proteins. Work in my lab is supported by the EPA Trust, Wellcome Trust and BBSRC.

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Authors and Affiliations

  1. Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK

    Stephen D. Bell

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  1. Stephen D. Bell
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Correspondence to Stephen D. Bell .

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  1. , School of Biology, University of St Andrews, Biomedical Sciences Research Complex, North Haugh, St Andrews, KY16 9ST, Fife, United Kingdom

    Stuart MacNeill

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Bell, S.D. (2012). Archaeal Orc1/Cdc6 Proteins. In: MacNeill, S. (eds) The Eukaryotic Replisome: a Guide to Protein Structure and Function. Subcellular Biochemistry, vol 62. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4572-8_4

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