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. 2024 Jan-Feb;71(1):e13003.
doi: 10.1111/jeu.13003. Epub 2023 Oct 6.

Kaonashia insperata gen. et sp. nov., a eukaryotrophic flagellate, represents a novel major lineage of heterotrophic stramenopiles

Elizabeth J Weston  1 , Yana Eglit  2 , Alastair G B Simpson  1
Affiliations

Kaonashia insperata gen. et sp. nov., a eukaryotrophic flagellate, represents a novel major lineage of heterotrophic stramenopiles

Elizabeth J Weston et al. J Eukaryot Microbiol. 2024 Jan-Feb.

Abstract

Eukaryotrophic protists are ecologically significant and possess characteristics key to understanding the evolution of eukaryotes; however, they remain poorly studied, due partly to the complexities of maintaining predator-prey cultures. Kaonashia insperata, gen. nov., et sp. nov., is a free-swimming biflagellated eukaryotroph with a conspicuous ventral groove, a trait observed in distantly related lineages across eukaryote diversity. Di-eukaryotic (predator-prey) cultures of K. insperata with three marine algae (Isochrysis galbana, Guillardia theta, and Phaeodactylum tricornutum) were established by single-cell isolation. Growth trials showed that the studied K. insperata clone grew particularly well on G. theta, reaching a peak abundance of 1.0 × 105 ± 4.0 × 104 cells ml-1 . Small-subunit ribosomal DNA phylogenies infer that K. insperata is a stramenopile with moderate support; however, it does not fall within any well-defined phylogenetic group, including environmental sequence clades (e.g. MASTs), and its specific placement remains unresolved. Electron microscopy shows traits consistent with stramenopile affinity, including mastigonemes on the anterior flagellum and tubular mitochondrial cristae. Kaonashia insperata may represent a novel major lineage within stramenopiles, and be important for understanding the evolutionary history of the group. While heterotrophic stramenopile flagellates are considered to be predominantly bacterivorous, eukaryotrophy may be relatively widespread amongst this assemblage.

Keywords: electron microscopy; evolution; heterokont; heterotroph; mastigonemes; phylogenetics; predator; protist.

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References

REFERENCES

    1. Adl, S.M., Bass, D., Lane, C.E., Lukeš, J., Schoch, C.L., Smirnov, A. et al. (2019) Revisions to the classification, nomenclature, and diversity of eukaryotes. The Journal of Eukaryotic Microbiology, 66, 4-119. Available from: https://doi.org/10.1111/jeu.12691
    1. Aleoshin, V.V., Mylnikov, A.P., Mirzaeva, G.S., Mikhailov, K.V. & Karpov, S.A. (2016) Heterokont predator Develorapax marinus gen. et sp. nov. - a model of the ochrophyte ancestor. Frontiers in Microbiology, 7, 1194. Available from: https://doi.org/10.3389/fmicb.2016.01194
    1. Andersen, R.A., Berges, F.A., Harrison, P.F. & Watanabe, M.M. (2005) f/2 media. In: Algal culturing techniques. Burlington, MA: Elsevier Science Publishing Co., p. 507.
    1. Andersen, R.A., Saunders, G.W., Paskind, M.P. & Sexton, J.P. (1993) Ultrastructure and 18S rRNA gene sequence for Pelagomonas calceolata gen. et sp. nov. and the description of a new algal class, the Pelagophyceae classis nov. Journal of Phycology, 29, 701-715.
    1. Belcher, J.H. (1975) The fine structure of the lorcicate colorless flagellate, Bicoeca planctonica Kisselaw. Archiv für Protistenkunde, 117, 78-84.

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