Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1996 Oct 15;98(8):1906-17.
doi: 10.1172/JCI118992.

Ablation of the murine alpha myosin heavy chain gene leads to dosage effects and functional deficits in the heart

W K Jones  1 , I L Grupp, T Doetschman, G Grupp, H Osinska, T E Hewett, G Boivin, J Gulick, W A Ng, J Robbins
Affiliations

Ablation of the murine alpha myosin heavy chain gene leads to dosage effects and functional deficits in the heart

W K Jones et al. J Clin Invest. .

Abstract

The alpha-myosin heavy chain (alpha-MyHC) is the major contractile protein expressed in the myocardium of adult mice. We have produced mice carrying a null mutation of alpha-MyHC by homologous recombination in murine ES cells. Homozygous null animals die between 11 and 12 d in utero of gross heart defects, while alpha-MyHC+/- heterozygotes survive and appear externally normal. The presence of a single functional alpha-MyHC+ allele in heterozygous animals results in reduced levels of the transcript and protein as well as fibrosis and alterations in sarcomeric structure. Examination of heart function using a working heart preparation revealed severe impairment of both contractility and relaxation in a subset of the alpha-MyHC+/- animals. Thus, two alpha-MyHC+ alleles are necessary for normal cardiac development, and hemizygosity for the normal allele can result in altered cardiac function.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Science. 1986 Feb 7;231(4738):597-600 - PubMed
    1. J Mol Cell Cardiol. 1994 Mar;26(3):361-8 - PubMed
    1. J Cell Biol. 1988 Dec;107(6 Pt 2):2613-21 - PubMed
    1. Genes Dev. 1989 Feb;3(2):131-40 - PubMed
    1. Dev Biol. 1990 Apr;138(2):348-58 - PubMed

Publication types

Substances