Hi,

I used Bambu to quantify transcripts based on a modified reference genome (because I did a allelic specific analysis and modified the official assembly). I faced the problem as below:

Error: BiocParallel errors
1 remote errors, element index: 1
0 unevaluated and other errors
first remote error:
Error in value[3L]: record 686186 (16:79451803-79451804) contains invalid DNA letters
file: .../hap2.fa
Execution halted

I have gone through the websites and someone mentioned it being due to R object GRanges. Would you please give a clue how to bypass or solve this issue?

Best regards
Sang