PKC epsilon is associated with myosin IIA and actin in fibroblasts
- PMID: 11897493
- DOI: 10.1016/s0898-6568(01)00277-7
PKC epsilon is associated with myosin IIA and actin in fibroblasts
Abstract
Proteins coimmunoprecipitating with protein kinase C (PKC) epsilon in fibroblasts were identified through matrix-assisted laser desorption/ionisation time of flight mass spectrometry (MALDI TOF m/s). This method identified myosin IIA in PKC epsilon immunoprecipitates, as well as known PKC epsilon binding proteins, actin, beta'Cop and cytokeratin. Myosin is not a substrate for PKC epsilon. Immunofluorescence analysis showed that PKC epsilon is colocalised with actin and myosin in actomyosin stress fibers in fibroblasts. Inhibitors of PKC and myosin ATPase activity, as well as microfilament-disrupting drugs, all inhibited spreading of fibroblasts after passage, suggesting a role for a PKC epsilon-actin-myosin complex in cell spreading.
Similar articles
-
Changes in protein kinase C epsilon phosphorylation status and intracellular localization as 3T3 and 3T6 fibroblasts grow to confluency and quiescence: a role for phosphorylation at ser-729?Biochem J. 2000 Nov 15;352 Pt 1(Pt 1):19-26. Biochem J. 2000. PMID: 11062054 Free PMC article.
-
Phorbol ester-induced translocation of PKC epsilon to the nucleus in fibroblasts: identification of nuclear PKC epsilon-associating proteins.FEBS Lett. 2004 Jul 16;570(1-3):20-4. doi: 10.1016/j.febslet.2004.05.080. FEBS Lett. 2004. PMID: 15251432
-
Molecular analysis of the interactions between protein kinase C-epsilon and filamentous actin.J Biol Chem. 1998 Oct 9;273(41):26790-8. doi: 10.1074/jbc.273.41.26790. J Biol Chem. 1998. PMID: 9756923
-
PKC-epsilon regulates basolateral endocytosis in human T84 intestinal epithelia: role of F-actin and MARCKS.Am J Physiol. 1999 Dec;277(6):C1239-49. doi: 10.1152/ajpcell.1999.277.6.C1239. Am J Physiol. 1999. PMID: 10600776
-
Protein kinase C mediation of Ca(2+)-independent contractions of vascular smooth muscle.Biochem Cell Biol. 1996;74(4):485-502. doi: 10.1139/o96-053. Biochem Cell Biol. 1996. PMID: 8960355 Review.
Cited by
-
Protein kinase C epsilon and genetic networks in osteosarcoma metastasis.Cancers (Basel). 2013 Apr 8;5(2):372-403. doi: 10.3390/cancers5020372. Cancers (Basel). 2013. PMID: 24216982 Free PMC article.
-
Sailing to and Docking at the Immune Synapse: Role of Tubulin Dynamics and Molecular Motors.Front Immunol. 2018 May 30;9:1174. doi: 10.3389/fimmu.2018.01174. eCollection 2018. Front Immunol. 2018. PMID: 29910809 Free PMC article. Review.
-
Human thrombopoiesis depends on Protein kinase Cδ/protein kinase Cε functional couple.Haematologica. 2016 Jul;101(7):812-20. doi: 10.3324/haematol.2015.137984. Epub 2016 Apr 14. Haematologica. 2016. PMID: 27081176 Free PMC article.
-
The Multifunctional Protein Kinase C-ε in Cancer Development and Progression.Cancers (Basel). 2014 Apr 10;6(2):860-78. doi: 10.3390/cancers6020860. Cancers (Basel). 2014. PMID: 24727247 Free PMC article.
-
Keratin 1 plays a critical role in golgi localization of core 2 N-acetylglucosaminyltransferase M via interaction with its cytoplasmic tail.J Biol Chem. 2015 Mar 6;290(10):6256-69. doi: 10.1074/jbc.M114.618702. Epub 2015 Jan 20. J Biol Chem. 2015. PMID: 25605727 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
