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. 2003 Apr 11;327(5):919-23.
doi: 10.1016/s0022-2836(03)00239-0.

How reliable are experimental protein-protein interaction data?

Einat Sprinzak  1 , Shmuel Sattath, Hanah Margalit
Affiliations

How reliable are experimental protein-protein interaction data?

Einat Sprinzak et al. J Mol Biol. .

Abstract

Data of protein-protein interactions provide valuable insight into the molecular networks underlying a living cell. However, their accuracy is often questioned, calling for a rigorous assessment of their reliability. The computation offered here provides an intelligible mean to assess directly the rate of true positives in a data set of experimentally determined interacting protein pairs. We show that the reliability of high-throughput yeast two-hybrid assays is about 50%, and that the size of the yeast interactome is estimated to be 10,000-16,600 interactions.

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