Complete atomic model of the bacterial flagellar filament by electron cryomicroscopy
- PMID: 12904785
- DOI: 10.1038/nature01830
Complete atomic model of the bacterial flagellar filament by electron cryomicroscopy
Abstract
The bacterial flagellar filament is a helical propeller for bacterial locomotion. It is a helical assembly of a single protein, flagellin, and its tubular structure is formed by 11 protofilaments in two distinct conformations, L- and R-type, for supercoiling. The X-ray crystal structure of a flagellin fragment lacking about 100 terminal residues revealed the protofilament structure, but the full filament structure is still essential for understanding the mechanism of supercoiling and polymerization. Here we report a complete atomic model of the R-type filament by electron cryomicroscopy. A density map obtained from image data up to 4 A resolution shows the feature of alpha-helical backbone and some large side chains. The atomic model built on the map reveals intricate molecular packing and an alpha-helical coiled coil formed by the terminal chains in the inner core of the filament, with its intersubunit hydrophobic interactions having an important role in stabilizing the filament.
Similar articles
-
Building the atomic model for the bacterial flagellar filament by electron cryomicroscopy and image analysis.Structure. 2005 Mar;13(3):407-12. doi: 10.1016/j.str.2005.02.003. Structure. 2005. PMID: 15766542
-
Structure of the bacterial flagellar protofilament and implications for a switch for supercoiling.Nature. 2001 Mar 15;410(6826):331-7. doi: 10.1038/35066504. Nature. 2001. PMID: 11268201
-
Divergence of quaternary structures among bacterial flagellar filaments.Science. 2008 Apr 18;320(5874):382-5. doi: 10.1126/science.1155307. Science. 2008. PMID: 18420936
-
[Electron cryomicroscopy of the bacterial flagellar nanomachine].Tanpakushitsu Kakusan Koso. 2004 Mar;49(4):534-42. Tanpakushitsu Kakusan Koso. 2004. PMID: 15024844 Review. Japanese. No abstract available.
-
The archaeabacterial flagellar filament: a bacterial propeller with a pilus-like structure.J Mol Microbiol Biotechnol. 2006;11(3-5):208-20. doi: 10.1159/000094055. J Mol Microbiol Biotechnol. 2006. PMID: 16983196 Review.
Cited by
-
Three-dimensional reconstruction of helical polymers.Arch Biochem Biophys. 2015 Sep 1;581:54-8. doi: 10.1016/j.abb.2015.04.004. Epub 2015 Apr 22. Arch Biochem Biophys. 2015. PMID: 25912526 Free PMC article. Review.
-
Allelic variation in two distinct Pseudomonas syringae flagellin epitopes modulates the strength of plant immune responses but not bacterial motility.New Phytol. 2013 Nov;200(3):847-860. doi: 10.1111/nph.12408. Epub 2013 Jul 19. New Phytol. 2013. PMID: 23865782 Free PMC article.
-
Convergent evolution in the supercoiling of prokaryotic flagellar filaments.Cell. 2022 Sep 15;185(19):3487-3500.e14. doi: 10.1016/j.cell.2022.08.009. Epub 2022 Sep 2. Cell. 2022. PMID: 36057255 Free PMC article.
-
Expression, purification, crystallization and preliminary X-ray diffraction analysis of a core fragment of FlgG, a bacterial flagellar rod protein.Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 May 1;69(Pt 5):547-50. doi: 10.1107/S1744309113008075. Epub 2013 Apr 30. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013. PMID: 23695574 Free PMC article.
-
Cryo-electron tomography of bacteria: progress, challenges and future prospects.Nat Rev Microbiol. 2009 Sep;7(9):666-75. doi: 10.1038/nrmicro2183. Epub 2009 Aug 10. Nat Rev Microbiol. 2009. PMID: 19668224 Free PMC article. Review.
MeSH terms
Substances
Associated data
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources
