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. 1991 Oct 21;291(2):245-8.
doi: 10.1016/0014-5793(91)81294-i.

Molecular cloning and sequence analysis of cDNA and genomic DNA for the human cone transducin alpha subunit

M Kubo  1 , T Hirano, M Kakinuma
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Free article

Molecular cloning and sequence analysis of cDNA and genomic DNA for the human cone transducin alpha subunit

M Kubo et al. FEBS Lett. .
Free article

Abstract

A novel GTP binding protein (G protein) alpha subunit cDNA was isolated from a T cell leukemia cell line, Jurkat, utilizing polymerase chain reaction (PCR). The predicted amino acid sequence of this G protein alpha subunit showed the highest identity (96.6%) to bovine cone cell-specific transducin (Tc alpha). The organization of the coding region of this G protein alpha subunit gene was composed of 8 exons and 7 introns. Northern hybridization revealed the presence of this G protein message in a retinoblastoma cell line, Y79. In Jurkat, however, the message was detectable only by reverse transcription/PCR. Taken together, this novel G protein alpha subunit must be human Tc.

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