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. 2009 Sep 15;392(2):180-2.
doi: 10.1016/j.ab.2009.05.033. Epub 2009 May 27.

Analysis of protein interactions with two-hybrid system in cultured insect cells

Hiroaki Mon  1 , Ryohei Sugahara, Sun-Mee Hong, Jae-Man Lee, Yusuke Kamachi, Yutaka Kawaguchi, Takahiro Kusakabe
Affiliations

Analysis of protein interactions with two-hybrid system in cultured insect cells

Hiroaki Mon et al. Anal Biochem. .

Abstract

Many biological processes are usually coupled to the formation of protein complexes. The yeast two-hybrid system is a powerful tool for analyzing protein-protein interactions. Different patterns of protein modifications, such as glycosylation, phosphorylation, and acetylation, may affect the ability of proteins to interact. In this study, we developed the two-hybrid system that can be used in insect cells. To validate the insect two-hybrid (I2H) system, we analyzed and confirmed the known oligomer or dimer formation of silkworm Rad51 or RPA2-RPA3, respectively. The results established the feasibility of the I2H system for efficient analysis of protein interaction under conditions that closely reflect the normal physiological environment.

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