Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel
- PMID: 9856938
- DOI: 10.1126/science.282.5397.2220
Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel
Abstract
Mechanosensitive ion channels play a critical role in transducing physical stresses at the cell membrane into an electrochemical response. The MscL family of large-conductance mechanosensitive channels is widely distributed among prokaryotes and may participate in the regulation of osmotic pressure changes within the cell. In an effort to better understand the structural basis for the function of these channels, the structure of the MscL homolog from Mycobacterium tuberculosis was determined by x-ray crystallography to 3.5 angstroms resolution. This channel is organized as a homopentamer, with each subunit containing two transmembrane alpha helices and a third cytoplasmic alpha helix. From the extracellular side, a water-filled opening approximately 18 angstroms in diameter leads into a pore lined with hydrophilic residues which narrows at the cytoplasmic side to an occluded hydrophobic apex that may act as the channel gate. This structure may serve as a model for other mechanosensitive channels, as well as the broader class of pentameric ligand-gated ion channels exemplified by the nicotinic acetylcholine receptor.
Similar articles
-
Structure and function of the bacterial mechanosensitive channel of large conductance.Protein Sci. 1999 Oct;8(10):1915-21. doi: 10.1110/ps.8.10.1915. Protein Sci. 1999. PMID: 10548036 Free PMC article. Review.
-
'Feeling the pressure': structural insights into a gated mechanosensitive channel.Curr Opin Struct Biol. 1999 Aug;9(4):448-54. doi: 10.1016/S0959-440X(99)80063-3. Curr Opin Struct Biol. 1999. PMID: 10449367 Review.
-
Structural models of the MscL gating mechanism.Biophys J. 2001 Aug;81(2):917-36. doi: 10.1016/S0006-3495(01)75751-7. Biophys J. 2001. PMID: 11463635 Free PMC article.
-
Structural determinants of MscL gating studied by molecular dynamics simulations.Biophys J. 2001 May;80(5):2074-81. doi: 10.1016/S0006-3495(01)76181-4. Biophys J. 2001. PMID: 11325711 Free PMC article.
-
Phosphatidylinositol is crucial for the mechanosensitivity of Mycobacterium tuberculosis MscL.Biochemistry. 2013 Aug 13;52(32):5415-20. doi: 10.1021/bi400790j. Epub 2013 Aug 1. Biochemistry. 2013. PMID: 23875651 Free PMC article.
Cited by
-
Membrane protein insertion and assembly by the bacterial holo-translocon SecYEG-SecDF-YajC-YidC.Biochem J. 2016 Oct 1;473(19):3341-54. doi: 10.1042/BCJ20160545. Epub 2016 Jul 19. Biochem J. 2016. PMID: 27435098 Free PMC article.
-
The principles and promising future of sonogenetics for precision medicine.Theranostics. 2024 Aug 12;14(12):4806-4821. doi: 10.7150/thno.98476. eCollection 2024. Theranostics. 2024. PMID: 39239514 Free PMC article. Review.
-
The gating mechanism of the bacterial mechanosensitive channel MscL revealed by molecular dynamics simulations: from tension sensing to channel opening.Channels (Austin). 2012 Jul-Aug;6(4):317-31. doi: 10.4161/chan.21895. Channels (Austin). 2012. PMID: 23146938 Free PMC article.
-
Mechanical coupling of the multiple structural elements of the large-conductance mechanosensitive channel during expansion.Proc Natl Acad Sci U S A. 2015 Aug 25;112(34):10726-31. doi: 10.1073/pnas.1503202112. Epub 2015 Aug 10. Proc Natl Acad Sci U S A. 2015. PMID: 26261325 Free PMC article.
-
Crystal structures of all-alpha type membrane proteins.Eur Biophys J. 2010 Apr;39(5):723-55. doi: 10.1007/s00249-009-0546-6. Epub 2009 Oct 14. Eur Biophys J. 2010. PMID: 19826804 Review.
Publication types
MeSH terms
Substances
Associated data
- Actions
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous
